What Is PCR Plate and about Material
There is a lot more to know about PCR consumables such as PCR tubes, PCR plates, real-time PCR consumables, and more. The following is a description of the relevant knowledge points of the PCR board by Hawach company.
What is a PCR plate:
PCR plate is a carrier, mainly used as a primer, Taq DNA polymerase, dNTP, template nucleic acid, Mg, buffer, etc. to participate in the amplification reaction in the polymerase chain reaction (Polymerase Chain Reaction, PCR).
PCR plate material:
- Its own material is now mainly polypropylene (PP), which can better adapt to the repeated high and low-temperature settings during the PCR reaction and can achieve high-temperature and high-pressure sterilization.
- In order to achieve high-throughput operations with guns, PCR instruments, etc.96 well PCR plates or 384-well PCR plates are commonly used.
- The plate type conforms to the SBS international standard. In order to adapt to the PCR instruments of different manufacturers, it can be divided into four design modes: no skirt, half skirt, raised skirt, and full skirt according to the skirt design.
Common colors of PCR plates:
There are transparent and white, among which white is more suitable for new real-time fluorescent quantitative PCR.
The use of PCR plates:
PCR plates used: in genetics, biochemistry, immunity, medicine, and other fields, and also for basic research such as gene isolation, cloning, nucleic acid sequence analysis, disease diagnosis, or any place with DNA and RNA.
Applicable instruments:
- 0.2ml PCR tube 12-row real-time PCR tube flat cap, ultra-thin wall
- 0.5ml transparent flat cap PCR single tube
- 0.2ml 8-strip real-time PCR tube cap, flat cap
- PCR consumables, PCR tubes
- 0.2ml 8-row ultra-thin transparent PCR tubes, colorless
- PCR tube 0.2ml transparent 12-row PCR tube convex cap, ultra-thin wall
- 200μl 96-well clear unskirted PCR plate
- 0.2ML transparent 8-row real-time fluorescence quantitative PCR tube flat cover without a heat source
- 0.2ml transparent 8-row PCR tube + cap, convex cap, ultra-thin wall
- 0.1ml clear 8-row real-time PCR tube with flat cap
- 0.2ml 8-row real-time PCR tube, flat cap, clear, ultra-thin wall
- PCR tube 0.2ml PCR single tube, thin wall, transparent, flat cap
- 0.2ml 8-row tube cap, dome cap (raised cap), colorless
Difference between PCR 96-well plate and 384-well plate:
1.qPCR 96-well plate:
- The ultra-thin-wall technology provides a heat conduction effect and promotes the full realization of sample amplification;
- There are letters and numbers vertically and horizontally, which are easy to mark, have good sealing, and prevent cross-contamination;
- Applicable to most automated experimental instruments;
- The original packaging plastic material is used, with no thermal decomposition products, and no endotoxin;
2.qPCR 384-well plate:
- PCR plate 384 with No DNase and RNase, no DNA and RNA;
- The ultra-thin-wall technology provides a heat conduction effect and promotes the full realization of sample amplification;
- The convex edge design of the tapered tube can effectively ensure the sealing and prevent cross-contamination;
- Applicable to most automated experimental instruments;
- The original packaging plastic material is used, with no thermal decomposition products, and no endotoxin;
There are more introduced about applications of PCR plates, how to choose PCR plates, etc.