Various Uses of Cell Culture Flasks

Cell culture flasks, as the name suggests, are used to culture cells. According to the material, cell culture flask material can be divided into glass and plastic, the bottom shape is square, rectangle, triangle, etc. Several uses of cell cultures flask are described below.

t25 cell culture flask cell culture flask vented cap

Brief introduction of cell culture flasks:

  • Glass and plastic material
  • According to the volume of the bottle, there are 5-500ml to choose from. In addition, after the surface is specially modified, the cells can be better adherent and cultured, which can be divided into 25-175cm2 according to the cell adhesion area.

Various uses of cell culture flasks:

Cell culture flask usage with different shapes:

  • Different shapes of culture flasks have different uses. For cell culture, a flat bottom is generally used, which is easy to observe under a microscope, the bottom area is clear, and the height of the cell culture medium is relatively consistent.
  • Therefore, when doing experiments, whether it is adherent cells or suspension cells, flat bottom plates are generally used. Be sure to use flat-bottom culture plates for absorbance measurements.
  • U-shaped or V-shaped is generally only used when there are special requirements.
  • For example, in immunology, when two different lymphocytes are cultured together, they need to be stimulated by contacting each other. At this time, a U-shaped plate is generally used, because the cells will be gathered in a small area due to the effect of gravity.
  • Round bottom flasks are also used for isotope incorporation experiments, which require cell harvesters to collect cell cultures, such as “mixed lymphocyte cultures”.
  • Type V is commonly used in cell killing and immune hemagglutination assays. This cell-killing assay can also be changed to a U-shape (after adding cells, centrifuge at low speed).

Cell culture flask usages with different caps :

  • Domestic cell culture flasks generally have closed caps without inner pads, which are often used for airtight culture to ensure air tightness.
  • There are many types of caps for imported culture flasks: cell culture flask vented caps, airtight caps, polyester caps, and septum caps. The performance of the airtight cover is the same as that of domestic products.
  • Polyester bottle caps are often for an open culture, and simply loosen the cap to exchange the gas in the bottle with the gas in the environment.
  • Commonly used in an open culture, recommended for CO2 incubator culture, especially for experiments that require long-term culture.
  • The septum cap has a pre-cut opening in the septum so a nozzle can be inserted through the septum to add fluids, aspirate, or harvest cells, reducing the chance of contamination and maintaining a closed and sterile environment for the cell culture flask.

There are more introduces about how to clean cell culture flasks, the types of cell culture flasks, etc.