Usage and Precautions of Petri Dishes

Petri dishes are glass instruments, mainly for microbial or cell culture. It consists of a flat disc-shaped bottom and a cover and is generally glass or plastic.

Petri dish use:

Glass Petri dishes are always for adherent cultures of plant material, microbial cultures, and animal cells.
Plastic Petri plates are polyethylene materials, which are disposable and multiple-use
And suitable for laboratory inoculation, streaking, and bacterial isolation operations, and can for the cultivation of plant materials.
Common specifications of Petri dishes: 35mm cell culture dish ,60mm, 75mm, 90mm, 100mm, 120mm, 150mm
Glass or plastic round vessels are mainly for holding liquid culture medium or solid agar medium for cell culture.
Mainly petri dish used in epidemic prevention stations, hospitals, biological products, the food industry, pharmaceutical industry
And other kinds of areas, the dishes are always for bacterial isolation and culture, the antibiotic titer testing
In agricultural aspects and other scientific research, the dishes are mainly for artificial cultivation and incubation of seeds, plants, and so on.
In the electronics industry or other industries, the dishes are used as utensils for qualitative testing and analysis

Precautions for the use of Petri dishes:

Petri dishes with culture medium:

All sizes, like Petri dishes 100 mm are fragile, so be careful when cleaning and handling.
It is best to clean the used petri dish in time and store it in a safe and fixed location to prevent damage and breakage.
If you use a petri dish with a diameter of 10cm, you should probably hold at least 10ml of culture medium.
If you add antibiotics to your culture medium, you don’t need to be particularly strict
Of course, a more rigorous operation is to open the lid of the petri dish with one hand (usually the left hand), hit an angle
And then use a long sterile pipette to suck the culture medium away, and then change the habit to add fresh medium.
When adding, try not to blow directly against the cells, blow against the sidewall of the dish, so that it flows into the petri dish.
Of course, it is good to be close to the alcohol lamp, but don’t be too close, the plastic is easy to be deformed by heat.
And just open the lid and directly turn the petri dish and pour the culture solution into the waste tank, and there is no pollution.
Whether it is changing the solution or other operations, the key is to try your best to do it with your hands.
Do not touch the mouth of any bottle or dish, keep it as far away as possible.
And lift the lid with one hand, hold the dish with one hand, then turn the bottom over, pour out the old medium
And then add new or washed it off with D-Hanks.