Usage and Precautions of Petri Dishes
Petri dishes are glass instruments, mainly for microbial or cell culture. It consists of a flat disc-shaped bottom and a cover and is generally glass or plastic.
Petri dish use:
- Glass Petri dishes are always for adherent cultures of plant material, microbial cultures, and animal cells.
- Plastic Petri plates are polyethylene materials, which are disposable and multiple-use
- And suitable for laboratory inoculation, streaking, and bacterial isolation operations, and can for the cultivation of plant materials.
- Common specifications of Petri dishes: 35mm cell culture dish ,60mm, 75mm, 90mm, 100mm, 120mm, 150mm
- Glass or plastic round vessels are mainly for holding liquid culture medium or solid agar medium for cell culture.
- Mainly petri dish used in epidemic prevention stations, hospitals, biological products, the food industry, pharmaceutical industry
- And other kinds of areas, the dishes are always for bacterial isolation and culture, the antibiotic titer testing
- In agricultural aspects and other scientific research, the dishes are mainly for artificial cultivation and incubation of seeds, plants, and so on.
- In the electronics industry or other industries, the dishes are used as utensils for qualitative testing and analysis
Precautions for the use of Petri dishes:
Petri dishes with culture medium:
- All sizes, like Petri dishes 100 mm are fragile, so be careful when cleaning and handling.
- It is best to clean the used petri dish in time and store it in a safe and fixed location to prevent damage and breakage.
- If you use a petri dish with a diameter of 10cm, you should probably hold at least 10ml of culture medium.
- If you add antibiotics to your culture medium, you don’t need to be particularly strict
- Of course, a more rigorous operation is to open the lid of the petri dish with one hand (usually the left hand), hit an angle
- And then use a long sterile pipette to suck the culture medium away, and then change the habit to add fresh medium.
- When adding, try not to blow directly against the cells, blow against the sidewall of the dish, so that it flows into the petri dish.
- Of course, it is good to be close to the alcohol lamp, but don’t be too close, the plastic is easy to be deformed by heat.
- And just open the lid and directly turn the petri dish and pour the culture solution into the waste tank, and there is no pollution.
- Whether it is changing the solution or other operations, the key is to try your best to do it with your hands.
- Do not touch the mouth of any bottle or dish, keep it as far away as possible.
- And lift the lid with one hand, hold the dish with one hand, then turn the bottom over, pour out the old medium
- And then add new or washed it off with D-Hanks.
There are more introduces about the use of Petri dishes in the laboratory, how to clean Petri dishes, etc.