The Difference between Serological Pipette and Graduated Pipette

Show some differences between the serological pipette and the graduated pipette, like different definitions, specifications, etc.

Differences between the serological pipette and graduated pipette:

The difference in the definition of serological pipettes and graduated pipettes:

50 ml serological pipette 10ml serological pipette

  • Define serological pipette as a fat belly straw, a single-marked straw.
  • Commonly serological pipette sizes have 1ml, 2ml, 5ml, 10ml, 15ml, 25ml, and 50ml (specially made separately), which are the first measuring instruments in quantitative experiments.
  • Graduated pipettes, that is, a glass tube with sub-gradations.
  • The commonly used graduated straws are 1ml, 2ml, 5ml, 10ml, and other specifications (specially made separately).

The difference in specifications of serological pipettes and graduated pipettes:

  •  People who use a serological pipette are limited, cause only has a few fixed specifications.
  • Contrary, People use the graduated pipette at their will at any time, so there is no limit to the graduated pipette.
  • And graduated pipette also depends on the accuracy required by your experiment. Generally, choosing a suitable graduated pipette for analysis experiments can also meet the requirements.

Difference between measuring in serological pipettes and graduated pipettes:  

  •  According to the defined serological pipette, which is a pipetting device to get a certain volume of solution with a pipette.
  •   The graduated pipette is a straight glass tube with graduation to know the volume of the liquid. So many people define it as a measuring instrument, which often makes for measuring the volume of the solution it releases.
  •  According to the standard, the type mark: measuring in type: In; measuring out type: Ex; blowing out type: Blow out.

The difference between using methods in serological pipettes and graduated pipettes:  

  • Before pipetting the solution with a pipette or graduated pipette, first, use a clean filter paper to absorb the water inside and outside the tip-end,
  • Then rinse with the solution to be piped 2-3 times to ensure that the concentration of the pipetted solution remains unchanged.

serological pipette:

  • When pipetting the solution, insert the lower tip-end into the solution 1-2cm to suck the solution.
  • When the liquid level of the sucked solution rises above the mark, lift the pipette and remove the solution outside the end of the pipette.
  • According to the scale of the pipette, put the solution slowly down.
  • Then put the pipette into the container, which can receive the solution, with the end of the pipe still against the inner wall of the container.
  • At this time, the pipette is vertical, and the receiving vessel is tilted so that all the solution in the pipe naturally stays along the wall of the vessel.
  • After waiting for 10-15 seconds, take out the pipette.

Graduated pipettes:

Marked with four symbols: “Fast”, “A”, “B”, and “Blow”.

  1. Write “fast” or “B” to indicate:
    After you finished the liquid, wait three seconds, and the transferred liquid volume reaches the marked liquid volume.
  1. For the tube with “A”: higher accuracy, wait for 15 seconds to let the pipette leave the container wall after finishing the liquid.
  1. Writing “blowing” means: When finished dispensing, the remaining liquid column at the tip-end of the pipette needs to be blown into the container with an ear wash ball to reach the target volume.
  1. “A” tubes rarely have “blow”, and “blow” tubes are generally “B” or “quick” tubes

There are more introduces to serological pipettes: the types of serological pipettes, how to use a serological pipette, etc.