Selection and Use of Cell Culture Plates
As a common and important tool for culturing cells, cell culture well plates have various shapes, specifications, and uses.
The cell culture plate material is mainly PS and has a treated surface. Facilitates cell adherent growth and extension. Of course, there are also growth materials for planktonic cells, as well as low-binding surfaces.
- Are you also confused about how to choose a suitable culture plate?
- Do you worried about how to use the culture plate conveniently and correctly?
Here are some answers about cell culture well plate uses:
The difference and choice of flat-bottom and round-bottom (U-shaped and V-shaped) cell culture plates:
1) Adherent cells generally use flat-bottom culture plates.
2) Mostly used U-shaped cell culture plates for culture suspension cells.
3) Made V-type plates sometimes for immunological hemagglutination experiments.
Different types of cell culture plate use:
- People generally used U or V-shaped plates, when certain special requirements are required.
For example, in immunology, when two different lymphocytes are mixed and cultured, they need to be in contact with each other to stimulate.
- A U-shaped plate is more popular than a V-shaped, one because the cells will gather in a small range due to the action of gravity.
- When doing MTT and other experiments, both adherent and suspended cells are generally flat-bottomed.
- If it is to grow cells, usually use a flat bottom. And according to the material, the label “Tissue Culture (TC) Treated” is for growing cells.
- People often used round-bottomed ones for analysis, chemical reactions, or to preserve samples. Round-bottomed ones are usually used for analysis, chemical reactions, or to preserve samples.
- Round bottom is better to absorb the liquid cleanly, if you use a flat bottom, it is not easy to suck. However, if you want to measure absorbance, you must buy a flat bottom.
- For the experiment of isotope incorporation, many people used a round-bottom culture plate. It is necessary to use a cell harvester to collect the culture of cells, such as a “mixed lymphocyte culture”.
- Most cell cultures use flat-bottom culture plates, which are easy to observe under the microscope and have a clear bottom area, and a relatively consistent cell culture liquid level.
Collection of questions on cell culture plate uses:
Q: I see that the culture plate has 4, 6, 12, 24, 48, and 96 well culture plates in several sizes, but I don’t know which size to use in the experiment, please advise.
Answer: Answer: According to your specific experimental requirements, 6 wells are generally for flow, 96 wells are generally for MTT, and 24 wells are generally for cell slides! It depends on your experiment!
Q: Does anyone know what kind of culture plate the Terasaki plate is, and what is the difference between it and an ordinary cell culture plate?
Answer: Often used Terasaki plate in crystallographic research, and is easy for crystal observation and structural analysis.
There are more introduces about what is cell culture plate material, is the precaution of washing cell culture flasks, etc.