Quick to Know Application of Deep Well Plates

The main body of the deep well plates is polymer material polypropylene, which has good chemical corrosion resistance and can be sterilized at high temperatures. But people don’t understand its purpose, here briefly introduce it:

Definition of deep well plates:

96 deep well culture plate Sterile 96 deep well culture plate

  • Deep-well plates are laboratory plates as the basis of the appearance and size of ordinary micro-well plates (mainly 96-well deep-well plates and 24-well plates).
  • And increase the depth of the holes to increase the volume of each hole while maintaining length and width to SBS international specifications.
  • In other definitions: the most basic experimental instrument for placing test tubes to dry in chemical experiments is the deep well plate.
  • Deep well plates are actually some tiny holes, and the test tubes can be for vertically, and can also be for observing the reaction process. Inverting washed tubes will aid in drying.

Deep well plate used for:

corning deep well plate 24 deep well plate U bottom

  • Deep well plate reuse in  DNA detection, high-throughput reaction, storage, and transfer of samples antibody titer detection
  • Sample storage:
  • It can replace the traditional 1.5ml centrifuge tube to store samples. Neat storage, space-saving, large storage capacity, can withstand -80 ℃ refrigerator. Due to their excellent storage properties, deep well plates are also called storage plates.
  • Injection operation:
  • And various companies often placed automation equipment in the sample room of the automation equipment for sampling.
  • Compared with traditional sampling, it can not only double the number of samples placed in the sample chamber but also realize direct sampling after the samples are processed on the 96-well plates deep well, saving the tedious work of sampling and placing samples.
  • Other applications of deep well plate:
  • Genomic DNA extraction, plasmid DNA extraction, nucleic acid extraction, and purification of various samples.
  • High-throughput automated liquid operations can realize high-throughput operations such as protein precipitation, liquid extraction, animal tissues, bacteria, plants, soil, clinical samples, and yeast.

What should attention to when using the plate:

  • People often usually control the liquid volume in each well to 150 μL. If there is too much liquid, it is easy to overflow and cause pollution.
  • There is less liquid per well, so it evaporates quickly in the incubator. And design the inoculum should be in the middle of the plate. Add media to the surrounding wells to maintain humidity within the plate.
  • And dispersed the cells should be full when separating the plates, add a few blanks and then blow off, otherwise, the cell concentration will be different
  • The surrounding wells of the plate are usually blank and do not support cells. The reason is simply the effect of temperature gradients and water evaporation. The so-called “edge effect”!
  •  After adding the cells, shake the dee well plate to make the cells evenly distributed on the plate, otherwise, there will be more cells around and fewer in the middle!

There are also introduces about the use of PCR plates, the PCR plate volume, etc.