Precautions of PCR Tubes In Experiments

What is a PCR tube: with the highest grade of medical-grade polypropylene, PCR tubes achieve better thermal conductivity, fast response, and uniform thermal conductivity.

The key factors affecting the PCR reaction of PCR tubes:

• PCR instrument, enzymes, magnesium ions, buffers, primer annealing temperature, etc,
• Among these, the quality of PCR consumables and the temperature accuracy of PCR instruments are so close to the experimental results
• PCR tubes and PCR plates require extremely thin walls and height uniformity.
• The thickness of the PCR tube wall, and all volumes of the PCR tube, will affect the heat transfer and heat transfer rate.
• And affect the results of PCR, especially PCR (quantitative PCR) also including homogeneity and light transmittance of the tube.
• With good heat transfer, optical permeability, tolerance, and airtightness are often a priority in the selection of PCR tubes.

Precautions for experimental operation:

PCR tubes use precautions:

1. High-precision small-throughput molds produce PCR consumables to ensure the uniformity and synchronization of plastic raw materials in the mold.
2. Cross-contamination between samples is also one of the reasons- causes for false-positive reactions. And pay attention to all aspects of magnification.
3. Wear disposable gloves. If the reaction solution is accidentally spilled, change gloves immediately.
4. Use disposable tips. It is strictly forbidden to mix the PCR product analysis room with the pipette tip.
5. Not exposing the tip to the air for a long time to avoid aerosol pollution.
6. Avoid splashing the reaction liquid. To avoid this when opening the reaction tube, centrifuge the solution to collect the liquid at the bottom of the tube before opening the lid.
7. If it accidentally spills on gloves or on the table. , immediately change gloves and wipe the tabletop with dilute acid.

When importing PCR tubes to handle multiple samples:

1. Pcr tube used in multiple samples, prepare the reaction solution, first mix dNTPs, buffer, primers, and enzymes
2. And then divide the package, which can reduce the operation, avoid pollution, and improve the accuracy of the reaction.
3. Finally, add the reaction template, and cap the reaction tube tightly after adding.
4. Set yin and yang control and blank control during operation, which can verify the reliability of PCR reaction and assist in judging the reliability of the amplification system.
5. Whenever possible, use replaceable or autoclaved samplers. Since contaminated samplers with product aerosols or sample DNA, use replaceable or autoclaved samplers.
6. Samplers, at least during PCR operations, should dedicate and not interchangeably, especially not carried the samplers used for PCR product analysis carried over to the other two areas.
7. Repeat the experiment, verify the results, and draw conclusions with caution.

There are more introduces about the material of PCR tubes, how to choose different volumes of PCR tubes, etc.