Precautions for Cleaning Petri Dish
In many laboratories, there is a glass or plastic vessel consisting of a flat disc-shaped bottom and a lid, which is for cultivating or isolating bacteria, which is a petri dish. So how to clean Petri dishes?
What is a petri dish?
In terms of the material of Petri dishes:
- A Petri dish definition is a glass or plastic round vessel to hold a liquid culture medium or solid agar medium for cell culture.
- TC treated culture dishes are basically above two categories: plastic and glass.
- People use glass Petri dishes to grow plant material, microorganisms, and animal cells.
- The plastic is polyethylene material, which is suitable for laboratory inoculation, division, and isolation of bacteria,
- Sometimes can cultivate plant materials, and use them according to your own use.
- A Petri dish consists of a bottom and a lid. As chemical equipment is often for cultivating bacteria.
- Usually made of glass or plastic. Petri dishes are fragile and fragile, so be careful when cleaning and handling them.
How to clean Petri dishes:
When the Petri dishes are cleaned:
- Goes through four steps: soaking, rinsing, acid soaking, and cleaning.
- When cleaning, pay attention to soaking the utensils with water to soften and dissolve the attachments.
- Then soak in hydrochloric acid for a certain time, so that the utensils will not have a large amount of protein and grease attached to the inside of the utensils when used them.
- Finally, pay attention to maintaining the hygiene of the utensils to avoid the infection of some transmitted diseases.
- Because the lid of the petri dish can prevent microorganisms in the air from falling into it, partially opened the lid of the petri dish except for the alcohol lamp during inoculation.
- After use, must clean the petri dish in time and stored it in a safe and fixed position to prevent damage and breakage.
After the Petri dishes are cleaned:
It is important that the petri dish is upside-down, and it needs to be upside down for the following reasons:
- Prevent the evaporation of the water in the medium, especially when poured the medium, if poured less, it is easier to dry up, affecting the growth of microorganisms.
- Putting it upside down can prevent the colony from spreading to a certain extent so that a single colony can be formed, which is convenient for counting. When the bottom is facing down, the water in the petri dish will evaporate. When it rises to the lid, due to the low temperature, condensation often forms. This condensation may drip, causing the individual colonies below to be mixed up.
- The water in the medium should evaporate to form water droplets. If the water droplets gather on the medium plane, it will affect the growth of the colony. Therefore, to ensure the dryness of the medium should invert the culture.
- This is one point, and the other is that it is easy to take, big and small, and not destroy, and it also prevents foreign objects from falling on the medium.
There are some introduces about how to make marks on a Petri dish, the Petri dish use, etc.