How to Thaw Cells

Cell culture refers to simulating the living conditions of tissues or cells of the body, allowing it to continue to survive and grow in a petri dish. We can obtain more cells with the same traits to facilitate related research. Most of the cells are stored frozen, and then thawed to wake them up when they need to be cultured. General resuscitation and thawing requires a full manual operation, and careful and standardized operations are required to successfully complete cell resuscitation, protect the quality of cells, and ensure the progress. Next, let’s talk about how to thaw cells:

Preparation of Thawing Cells

  • When taking out the cryopreservation tube, take personal protection and wear gloves.
  • Ensure that the operating table is clean and sterile, avoiding unnecessary pollution that endangers the health of cells
  • Prepare the experimental equipment to be used, check the temperature of the water bath, the status of the centrifuge and centrifuge tubes, and sterile disposable gloves.
  • Preheat the medium to be used in advance.

How to Thaw Cells in Detail:

  • A Brief Introduction of Centrifuge TubesTake the cells in the cryopreservation tube out of the frozen storage place, and take it out quickly to avoid the influence of temperature difference on the cells. Cryotubes can be thawed in sterile disposable gloves to avoid contamination. Frozen cells should be immediately placed in a 37°C water bath for thawing after removal. The step of thawing should be fast and should be completed within 1 min, which is the key to recovering cells.
  • Wipe the outside of the cryovial with 70% ethanol and transfer it into a sterile operating table, and transfer the pre-warmed medium into it to slowly dilute the thawed cells. Slowly use a pipette gun to draw the liquid in the bottle into a 15ml centrifuge tube, then add an appropriate amount of medium, and mix gently.
  • Next is centrifugation: centrifugation speed and duration vary by cell type. After centrifugation, discard the supernatant, add fresh medium to resuspend the cells, and transfer them to a suitable culture container and culture environment.


  • Be careful of the damage caused by low temperature when taking out the frozen cells, and wear antifreeze gloves.
  • It is very important to resuscitate the cells quickly, try not to exceed one minute.
  • Try to maintain high density after thawing to improve cell survival rate.
  • Pay attention to aseptic operation and prevent cell contamination, which can help the cells to maintain a good state.

The above is all about how to thaw cell, if you have any questions about this, please contact us. If you need related experimental equipment, you can take a look at our high-quality cell culture flasks and centrifuge tubes. It is our company’s mission to provide guaranteed laboratory consumables for scientific researchers.