How to Install and Usage Pipette Tips

A complete pipetting cycle, including the steps of pipette tips installation – volume setting – suction and discharge – removal of tips. there are some steps that need to be followed.

How to install pipette tip:

1. Install pipette tips

For single-channel pipettes, insert the tip of the pipette vertically into the pipette tip, and then turn it slightly left and right to tighten it;
For multi-channel pipettes, align the channel of the pipette with the tips, insert it obliquely, and shake it back and forth to tighten it;
Make sure the airtightness of the tip, does not impact the pipette.

2. Capacity setting

Turn it counterclockwise to the scale to adjust from a large volume to a small volume;
When adjusting from a small volume to a large volume, you can first adjust the set volume clockwise, and then return to the set volume, which can ensure accuracy.
Do not turn the adjustment knob out of the range, otherwise, it will damage the mechanical device in the pipette.

3. Aspiration and discharge

Press the lab pipette tips button to the position, release the button, and perform aspiration.
Remember not to be too fast, otherwise, the liquid will enter the tip too fast, and suck the pipette.
The liquid is close to the wall of the container, first press the first gear, pause for a while, and then press the second gear to remove the remaining liquid.

4. Vertical suction.

For 5 and 10ul pipettes tips, immersed the tip in the liquid surface for 5mm, slowly suck the liquid,
After reaching the ideal volume, leave the liquid surface unless pause under the liquid surface for seconds
When sucking volatile liquid, it is necessary to rinse the suction head 4-6 times to make the steam in the sleeve chamber saturated, which can avoid liquid leakage.

Correct placement of the pipette and pipette tip:

After pipette tips use, you can hang it upright on the pipette holder, but not drop it.
Do not put the pipette horizontally or upside down, so as to avoid the liquid from flowing back and corroding the piston spring.
If not in use, adjust the range of the pipette to a large scale, so that the spring is in a relaxed state to protect the spring

Common mistakes of disposable pipette tips use:

When assembling the disposable pipette tips use, repeatedly hitting the tip makes it difficult to remove the tip and even damages the pipette.
When aspirating liquid, tilted the pipette, resulting in inaccurate pipetting, and the liquid can easily enter the pipette handle.
When aspirating liquid, releasing the thumb quickly will force the liquid to form a turbulent state, and the liquid will directly rush into the inside of the pipette.
Press directly to the second gear to aspirate (should follow the standard method above).
Use a large-scale pipette to remove small-volume samples (selected a pipette with a suitable range).
Lay the pipette with the residual liquid tip flat ( hung on the pipette rack).

There are also introduces about the function of disposable pipette tips, what is sterile filtered pipette tips, etc.