How to Choose the Right ELISA Plates
There are many kinds of ELISA plates, and people often do not know how to make Elisa plate, how to choose them? Today, show you the selection skills of the ELISA plates!
How to choose ELISA Plates:
One: According to the bottom of the ELISA plates
- ELISA plate is made up of a flat bottom, a U-shaped bottom, and V-shaped bottom
- The refractive index of the flat bottom is low, which is suitable for detection in the microplate reader;
- The refractive index of the ELISA plates with the U-shaped bottom is higher, which is convenient for operations such as adding and mixing.
- The V-bottomed ELISA plates can accurately aspirate samples.
Two: According to the design of the ELISA plates
1. Elisa plate design:
Elisa plate design with a high binding force, medium binding force, and amination
2. High binding capacity Elisa plate:
- After the surface is treated, the ELISA plate material is made up of PS.
- And high binding ELISA plate can improve the sensitivity, and can relatively reduce the concentration and dosage of the coated protein
- A disadvantage is easy to produce non-specific reactions.
- After antigen or antibody coating, non-ionic detergents cannot effectively block unbound protein sites.
- Different binding abilities of the ELISA plates to protein and other molecules
3. Moderate binding capacity Elisa plate:
- Passively bound to proteins via surface hydrophobic bonds,
- Due to the characteristics of a moderate binding ELISA plate that only binds to macromolecules, it is suitable for use as a solid-phase carrier for unpurified antibodies or antigens
- Moderate binding ELISA plate which can reduce potential non-specific cross-reactions.
- People use moderate binding ELISA plates to block with inert proteins or non-ionic detergents.
- Has a positively charged amino group
- This type is suitable as a solid-phase carrier for small molecular proteins. Using the appropriate buffer and pH,
- The surface can bind negatively charged small molecules via ionic bonds.
- Due to the hydrophilic nature of its surface and its ability to be covalently bound by other cross-linking agents, users used it to immobilize protein molecules dissolved in detergents.
- The disadvantage of the amination type is some protein molecules do not bind due to the reduced hydrophobicity;
- Due to the hydrophilic and covalent surface properties, the blocking solution used must interact with non-reactive amino groups and any functional groups in the chosen crosslinker.
Three: According to the color of the ELISA plates
- Divided into transparent, black, white color
- Commonly used and used for the most general ELISA experiments is transparent.
- The black ELISA plate itself will absorb light, so its signal is much lower than that of the white plate, so people used to detect strong light
- And used for weak light detection and is often used for general chemiluminescence in a white ELISA plate.
- In addition, the black microtiter plate can also reduce the problems caused by non-specific reactions.
- When the light is strong, the influence between adjacent wells will be great, so the chemiluminescence experiment cannot be performed with a transparent ELISA plate.