How to Add Samples and Manipulation in Cell Culture Plates
Cell culture plates also follow strict aseptic principles during cell manipulation. All operations should standardized and scientific, and will not cause an additional impact on cell growth. Here are some common questions about how to ensure the homogeneity of the cells after adding the sample.
How to add samples and manipulation in cell culture plates rightly:
- The surface area of cell culture plates in the incubator should clean (scrubbing with ultraviolet rays and alcohol regularly and switching on and off as little as possible)
- The humidity inside the incubator should maintain at 100% at all times (place a can of sterile distilled water in the incubator).
- In addition, just like a petri dish, it is also a container with an upside-down lid that won’t get contaminated.
- Mainly due to the negative air pressure generated by the “L”-shaped edge of the cover, microorganisms adhere to the dust
- And the dust carried by the airflow cannot pass through the edge of the cover that generating negative pressure.
- The ventilation effect is only through the diffusion of the air, no airflow is generated, so it only breathes and does not penetrate bacteria.
Some questions about the cell culture plate:
1. Bacteria in a cell culture plate：
Ask: The lid of a 24-well cell culture plate or dish is loose and ventilated, but can contaminants such as bacteria and mold slip in?
- The purpose of this is for respiration (actually to allow the adequate exchange of carbon dioxide outside the dish with the dish, maintaining the pH of the medium).
- Everything has advantages and disadvantages, which of course increases the possibility of contamination.
- And the lid is very loose and belongs to the half-open culture.
- This also evaporates the liquid in the petri dish, which is remarkable for precise drug dosing. Therefore, the following two measures are necessary
2. How to avoid polluting:
Using a 24-well plate cell culture, work in some wells (on the bench) and grow cells in others. I am worried about pollution, what should I be aware of?
- And make full use of the lid of the cell culture well plate version, try to expose only the holes manipulate,e and cover the other holes with lids.
- Take advantage of all the holes before use; if you only need a few holes, you can use only one side and cover the rest with a lid.
- And use the right hole first (the right hand is convenient for adding samples).
- In the ultra-clean bench, if the operation should standardize, it should be ok.
- And when working with cell culture plates, it is generally no problem to lift one side of the plate with a few slides without fully opening the lid.
Cell culture plates are for laboratory cell culture, and their excellent optical properties are convenient for microscope observation. And treated surface with TC and the cell adhesion effect is better. and minimize the effect of medium changes on the cell growth state.