Applications and Features of PCR Plates

There are many types of PCR consumables: 8 continuous tubes, low volume tubes, standard tubes, and a series of PCR tubes and qPCR plates.

Applications of PCR plates:

96 well PCR plate real time pcr 384 well plate

  • PCR plate uses in genetics, biochemistry, immunology, medicine, and other fields.
  • In addition, not only for basic research such as gene isolation, cloning, nucleic acid sequence analysis
  • And for the place with DNA and RNA, and disease diagnosis.
  • In order to achieve a high-throughput operation of guns and PCR instruments,  people used 96-well or 384-well PCR plates.
  • And PCR plates are important as primers in a polymerase chain reaction (PCR), Taq DNA polymerase, dNTPs, template nucleic acids, Mg, buffers, etc.

PCR plate features:

PCR plate material:

The material is now mainly polypropylene (PP)

PCR plate design:

  1. The PCR board above three design modes: no skirt, half skirt, and full skirt according to the skirt design.
  2. To better adapt to repeated high and low-temperature settings during the PCR reaction and can achieve high-temperature and high-pressure sterilization.
  3. And users design different cut corners or edge protrusions according to the real-time PCR instruments on the market.
  4. Sealing Films Pressure-sensitive adhesive cover films provide a seal for each well, reducing the chance of well-to-well contamination and sample evaporation, helping to ensure consistency of real-time PCR data, ease of use, and preventing loss of valuable samples.
  5.  And raised the edge of each hole to increase the sealing performance of the sealing plate and reduce the possibility of liquid volatilization.
  6. In addition, free of pyrogens, DNA/RNases, PCR inhibitors, and human genomic DNA.
  7. Using high-quality bio-polypropylene, produced white PCR plates in an ultra-clean production environment.
  8. And the surface is not easy to adhere to biomolecules and has good chemical resistance and temperature resistance
  9.  Pasteurize and can also withstand temperature changes during thermal cycling.
  10. And these materials are often in direct contact with reagents or samples, so often require high-quality materials and good processing techniques during production and preparation.
  11. And the thin tube wall, the wall thickness is uniform, and with efficiency the heat transfer
  12.  The frame of Hawach PCR plates is more rigid. The opening design ensures that the PCR reaction will not be deformed by quenching and heating.
  13. Well-matched heat transfer grooves to better promote heat transfer.
  14. And with high-precision mold manufacturing and precision plastic molding process to ensure product quality and batch-to-batch uniformity/stability.

There are more introduces about the use of PCR plates, the PCR plate volume, etc.